PrimePCR™ SYBR® Green Assay: Hist1h2bl, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hist1h2bl, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hist1h2bl, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0008974
Assay Design:   exonic
Chromosome Location:   17:45665692-45665863question
Amplicon Length:   142
Splice Variants Targeted:   ENSRNOT00000021095 ENSRNOT00000042077 ENSRNOT00000048218 ENSRNOT00000074737 ENSRNOT00000028779 ENSRNOT00000064705 ENSRNOT00000004347 ENSRNOT00000000824 ENSRNOT00000040740 ENSRNOT00000072195 ENSRNOT00000030198 ENSRNOT00000075811 ENSRNOT00000072293 ENSRNOT00000071688 ENSRNOT00000041423 ENSRNOT00000048465

Gene Information

somatic H2b histone that is replaced with a testis-specific form during spermatogenesis [RGD, Feb 2006]

Gene Symbol:   Hist1h2bl
Gene Name:   Histone H2B type 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050098
Entrez:   64647
Chromosome Mapping:   17q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.995500
y-intercept 35.330000
Efficiency 109

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download