PrimePCR™ SYBR® Green Assay: Foxi2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Foxi2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Foxi2, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0008626
Assay Design:   exonic
Chromosome Location:   1:214587391-214587599question
Amplicon Length:   179
Splice Variants Targeted:   ENSRNOT00000073222 ENSRNOT00000074792 ENSRNOT00000074699 ENSRNOT00000042693 ENSRNOT00000073754 ENSRNOT00000062058 ENSRNOT00000034630 ENSRNOT00000044505 ENSRNOT00000074130 ENSRNOT00000072076 ENSRNOT00000074598 ENSRNOT00000048946 ENSRNOT00000074402 ENSRNOT00000071037 ENSRNOT00000043877 ENSRNOT00000070942 ENSRNOT00000073032 ENSRNOT00000072232 ENSRNOT00000048588 ENSRNOT00000050241 ENSRNOT00000033032 ENSRNOT00000050133 ENSRNOT00000072360 ENSRNOT00000048913 ENSRNOT00000041332 ENSRNOT00000044270 ENSRNO

Gene Information

liver transcription factor that mediates the coordinate expression of hepatocyte-specific genes [RGD, Feb 2006]

Gene Symbol:   Foxi2
Gene Name:   Forkhead box protein I2
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000018917
Entrez:   246073
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.600000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download