PrimePCR™ SYBR® Green Assay: RGD1566189, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGD1566189, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1566189, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0008623
Assay Design:   exonic
Chromosome Location:   1:183063795-183064020question
Amplicon Length:   196
Splice Variants Targeted:   ENSRNOT00000002759 ENSRNOT00000067060 ENSRNOT00000061713 ENSRNOT00000032244 ENSRNOT00000001975 ENSRNOT00000048298 ENSRNOT00000012519 ENSRNOT00000010203 ENSRNOT00000075480 ENSRNOT00000073781 ENSRNOT00000024501 ENSRNOT00000072926 ENSRNOT00000061195 ENSRNOT00000074365 ENSRNOT00000073996 ENSRNOT00000009028 ENSRNOT00000010216 ENSRNOT00000021863 ENSRNOT00000022012 ENSRNOT00000024193 ENSRNOT00000051783 ENSRNOT00000006554 ENSRNOT00000025174 ENSRNOT00000044575 ENSRNOT00000049856 ENSRNOT00000044711 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1566189
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029082
Entrez:   499244
Chromosome Mapping:   1q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.210000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download