PrimePCR™ SYBR® Green Assay: Mef2a, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0008536
Assay Design:   exonic
Chromosome Location:   1:129308486-129332805question
Amplicon Length:   74
Splice Variants Targeted:   ENSRNOT00000041296 ENSRNOT00000041538 ENSRNOT00000047689 ENSRNOT00000070864 ENSRNOT00000072915

Gene Information

Description Not Available

Gene Symbol:   Mef2a
Gene Name:   Myocyte-specific enhancer factor 2A
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047756
Entrez:   309957
UniGene:   Rn.162435
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.100000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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