This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Myog, Rat
PrimePCR™ Template for SYBR® Green Assay: Myog, Rat
This gene belongs to the myogenic factor subfamily of the basic helix-loop-helix (BHLH) family of transcription factors. The protein encoded by this gene is a muscle-specific skeletal myogenin, which acts in concert with other family members to regulate myogenesis. The protein plays an essential role in the conversion of undifferentiated cells to myoblasts and functional skeletal muscle. [provided by RefSeq, Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.