PrimePCR™ SYBR® Green Assay: C1qbp, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0008291
Assay Design:   exonic
Chromosome Location:   10:57359215-57359361question
Amplicon Length:   117
Splice Variants Targeted:   ENSRNOT00000037517

Gene Information

binds to the globular heads of complement component C1q [RGD, Feb 2006]

Gene Symbol:   C1qbp
Gene Name:   Complement component 1 Q subcomponent-binding protein, mitochondrial
Aliases:   Not Available
RefSeq:   NM_019259
Ensembl:   ENSRNOG00000006949
Entrez:   29681
UniGene:   Rn.2765
Chromosome Mapping:   10q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.120000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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