PrimePCR™ SYBR® Green Assay: Tas2r119, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0008229
Assay Design:   exonic
Chromosome Location:   2:104978253-104978385question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000015904

Gene Information

member of a family of mammalian taste receptors that couple with the G protein alpha subunit gustducin; may play a role in bitter taste perception [RGD, Feb 2006]

Gene Symbol:   Tas2r119
Gene Name:   Taste receptor type 2 member 119
Aliases:   Not Available
RefSeq:   NM_023993
Ensembl:   ENSRNOG00000011963
Entrez:   78979
UniGene:   Rn.48782
Chromosome Mapping:   2q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.290000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download