PrimePCR™ SYBR® Green Assay: Ece2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007860
Assay Design:   exonic
Chromosome Location:   11:87021158-87021306question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000044482 ENSRNOT00000042833 ENSRNOT00000011571

Gene Information

metalloprotease type II enzyme; catalyzes functionally active forms of the endothelin vasoactive peptide family [RGD, Feb 2006]

Gene Symbol:   Ece2
Gene Name:   Endothelin-converting enzyme 2
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000001715
Entrez:   408243
UniGene:   Rn.169590
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.050000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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