PrimePCR™ SYBR® Green Assay: Syngap1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007822
Assay Design:   exonic
Chromosome Location:   20:7606260-7606349question
Amplicon Length:   60
Splice Variants Targeted:   ENSRNOT00000040859 ENSRNOT00000042332 ENSRNOT00000000572 ENSRNOT00000028015

Gene Information

acts as a Ras-GTPase activator; activity is inhibited by CaMKII phosphorylation; may inhibit NMDA receptor mediated MAPK signaling [RGD, Feb 2006]

Gene Symbol:   Syngap1
Gene Name:   Ras GTPase-activating protein SynGAP isoform 1
Aliases:   Not Available
RefSeq:   NM_181092 NM_001113409
Ensembl:   ENSRNOG00000000483
Entrez:   192117
UniGene:   Rn.9908
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.220000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download