PrimePCR™ SYBR® Green Assay: Hnf1a, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007796
Assay Design:   exonic
Chromosome Location:   12:49225951-49226050question
Amplicon Length:   70
Splice Variants Targeted:   ENSRNOT00000001565

Gene Information

acts as a transcriptional activator; plays a role in the regulation of liver-specific genes [RGD, Feb 2006]

Gene Symbol:   Hnf1a
Gene Name:   Hepatocyte nuclear factor 1-alpha
Aliases:   Not Available
RefSeq:   NM_012669
Ensembl:   ENSRNOG00000001183
Entrez:   24817
UniGene:   Rn.9660
Chromosome Mapping:   12q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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