PrimePCR™ SYBR® Green Assay: A2m, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007656
Assay Design:   exonic
Chromosome Location:   4:221442470-221442939question
Amplicon Length:   99
Splice Variants Targeted:   ENSRNOT00000019346

Gene Information

plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]

Gene Symbol:   A2m
Gene Name:   Alpha-2-macroglobulin
Aliases:   Not Available
RefSeq:   NM_012488
Ensembl:   ENSRNOG00000028896
Entrez:   24153
UniGene:   Rn.225884
Chromosome Mapping:   4q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.580000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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