PrimePCR™ SYBR® Green Assay: Ptx3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007647
Assay Design:   exonic
Chromosome Location:   2:177462221-177462368question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000016541

Gene Information

may play a role in protection against seizure-induced neurodegeneration [RGD, Feb 2006]

Gene Symbol:   Ptx3
Gene Name:   Pentraxin-related protein PTX3 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000012280
Entrez:   689388
UniGene:   Rn.218702
Chromosome Mapping:   2q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.250000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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