PrimePCR™ SYBR® Green Assay: Gpt, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007641
Assay Design:   exonic
Chromosome Location:   7:117747118-117747259question
Amplicon Length:   112
Splice Variants Targeted:   ENSRNOT00000050556

Gene Information

human homolog is a cytosolic alanine aminotransferase [RGD, Feb 2006]

Gene Symbol:   Gpt
Gene Name:   Alanine aminotransferase 1
Aliases:   Not Available
RefSeq:   NM_031039
Ensembl:   ENSRNOG00000033915
Entrez:   81670
UniGene:   Rn.6318
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 37.250000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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