PrimePCR™ SYBR® Green Assay: Camk2g, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007382
Assay Design:   exonic
Chromosome Location:   15:3669228-3682564question
Amplicon Length:   72
Splice Variants Targeted:   ENSRNOT00000066163 ENSRNOT00000065644 ENSRNOT00000067162 ENSRNOT00000041533

Gene Information

gamma chain of the CaM kinase II; involved in regulation of potassium currents, particularly in response to oxidative stress [RGD, Feb 2006]

Gene Symbol:   Camk2g
Gene Name:   Calcium/calmodulin-dependent protein kinase type II subunit gamma
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000009783
Entrez:   171140
UniGene:   Rn.10961
Chromosome Mapping:   15p16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.650000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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