PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000049360, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000049360, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000049360, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007249
Assay Design:   exonic
Chromosome Location:   10:44268421-44268565question
Amplicon Length:   115
Splice Variants Targeted:   ENSRNOT00000075040 ENSRNOT00000050288 ENSRNOT00000071834 ENSRNOT00000072352 ENSRNOT00000071556 ENSRNOT00000043430 ENSRNOT00000047914 ENSRNOT00000073040 ENSRNOT00000047671 ENSRNOT00000044332 ENSRNOT00000071262 ENSRNOT00000075204 ENSRNOT00000072854 ENSRNOT00000072282 ENSRNOT00000048778 ENSRNOT00000074062

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000049360
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049360
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.910000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download