PrimePCR™ SYBR® Green Assay: Brd2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007133
Assay Design:   exonic
Chromosome Location:   20:7329793-7329942question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000000535

Gene Information

mouse homolog may act as a transcriptional regulator, possibly involved in regulation of mammary epithelial cell proliferation and lactation [RGD, Feb 2006]

Gene Symbol:   Brd2
Gene Name:   Bromodomain-containing protein 2
Aliases:   Not Available
RefSeq:   NM_212495
Ensembl:   ENSRNOG00000000461
Entrez:   294276
UniGene:   Rn.98146
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.390000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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