PrimePCR™ SYBR® Green Assay: Map3k8, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0007030
Assay Design:   exonic
Chromosome Location:   17:54150688-54150824question
Amplicon Length:   107
Splice Variants Targeted:   ENSRNOT00000022190

Gene Information

kinase; involved in signaling pathways for cell proliferation/differentiation and stress responses [RGD, Feb 2006]

Gene Symbol:   Map3k8
Gene Name:   Mitogen-activated protein kinase kinase kinase 8
Aliases:   Not Available
RefSeq:   NM_053847
Ensembl:   ENSRNOG00000016378
Entrez:   116596
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download