PrimePCR™ SYBR® Green Assay: Mrgprb4, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006911
Assay Design:   exonic
Chromosome Location:   1:104488823-104488970question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000045792

Gene Information

seven transmembrane domain G-protein coupled receptor; most often found in sensory neurons [RGD, Feb 2006]

Gene Symbol:   Mrgprb4
Gene Name:   Mas-related G-protein coupled receptor member B4
Aliases:   Not Available
RefSeq:   NM_001002287
Ensembl:   ENSRNOG00000033021
Entrez:   404658
UniGene:   Rn.155107
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.870000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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