PrimePCR™ SYBR® Green Assay: Slc17a8, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006892
Assay Design:   exonic
Chromosome Location:   7:30371432-30371538question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000010207

Gene Information

has glutamate transporter activity; may play a role in glutamate transport in synaptic vesicles of cholinergic and serotoninergic neurons [RGD, Feb 2006]

Gene Symbol:   Slc17a8
Gene Name:   Vesicular glutamate transporter 3
Aliases:   Not Available
RefSeq:   NM_153725
Ensembl:   ENSRNOG00000007581
Entrez:   266767
UniGene:   Rn.84876
Chromosome Mapping:   7q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 36.190000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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