PrimePCR™ SYBR® Green Assay: Bpgm, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006851
Assay Design:   exonic
Chromosome Location:   4:61678650-61678797question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000013569

Gene Information

human homolog is a trifunctional enzyme which displays synthase, mutase, and phosphatase activities [RGD, Feb 2006]

Gene Symbol:   Bpgm
Gene Name:   Bisphosphoglycerate mutase
Aliases:   Not Available
RefSeq:   NM_199382
Ensembl:   ENSRNOG00000010133
Entrez:   296973
UniGene:   Rn.204528
Chromosome Mapping:   4q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.550000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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