PrimePCR™ SYBR® Green Assay: Dfna5, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006655
Assay Design:   exonic
Chromosome Location:   4:144606600-144606743question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000013264

Gene Information

mutation of this gene in humans is associated with autosomal dominant nonsyndromic hearing impairment [RGD, Feb 2006]

Gene Symbol:   Dfna5
Gene Name:   Deafness, autosomal dominant 5
Aliases:   Not Available
RefSeq:   NM_001191749
Ensembl:   ENSRNOG00000009970
Entrez:   353316
UniGene:   Rn.96433
Chromosome Mapping:   4q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.660000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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