PrimePCR™ SYBR® Green Assay: Mbd1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006546
Assay Design:   exonic
Chromosome Location:   18:69404277-69404387question
Amplicon Length:   81
Splice Variants Targeted:   ENSRNOT00000032202

Gene Information

Description Not Available

Gene Symbol:   Mbd1
Gene Name:   Methyl-CpG-binding domain protein 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000024104
Entrez:   291439
UniGene:   Rn.3310
Chromosome Mapping:   18q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.370000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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