PrimePCR™ SYBR® Green Assay: Slc22a6, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006506
Assay Design:   exonic
Chromosome Location:   1:231767120-231767230question
Amplicon Length:   81
Splice Variants Targeted:   ENSRNOT00000024757

Gene Information

multispecific organic anion transporter; involved in anion transport in a proximal tubule in the kidney [RGD, Feb 2006]

Gene Symbol:   Slc22a6
Gene Name:   Solute carrier family 22 member 6
Aliases:   Not Available
RefSeq:   NM_017224
Ensembl:   ENSRNOG00000018215
Entrez:   29509
UniGene:   Rn.87849
Chromosome Mapping:   1q43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.030000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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