PrimePCR™ SYBR® Green Assay: C9, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006122
Assay Design:   exonic
Chromosome Location:   2:75528107-75528818question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000018545

Gene Information

component of the terminal complement complex C5b-9, which induces cleavage and activation of caspase 3 and mediates induction of apoptosis [RGD, Feb 2006]

Gene Symbol:   C9
Gene Name:   Complement component C9 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000013736
Entrez:   117512
UniGene:   Rn.10252
Chromosome Mapping:   2q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.710000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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