PrimePCR™ SYBR® Green Assay: Add2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006046
Assay Design:   exonic
Chromosome Location:   4:182418027-182421561question
Amplicon Length:   90
Splice Variants Targeted:   ENSRNOT00000021491

Gene Information

plays a role in membrane ion transport; variation is associated with blood pressure variation in the Milan hypertensive strain [RGD, Feb 2006]

Gene Symbol:   Add2
Gene Name:   Beta-adducin isoform a
Aliases:   Not Available
RefSeq:   NM_001109880 NM_012491
Ensembl:   ENSRNOG00000015903
Entrez:   24171
UniGene:   Rn.11353
Chromosome Mapping:   4q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.100000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download