PrimePCR™ SYBR® Green Assay: Ruvbl1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005946
Assay Design:   exonic
Chromosome Location:   4:185676666-185676813question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000018339

Gene Information

nuclear DNA helicase that binds TBP; involved in recombination and transcription [RGD, Feb 2006]

Gene Symbol:   Ruvbl1
Gene Name:   RuvB-like 1
Aliases:   Not Available
RefSeq:   NM_147177
Ensembl:   ENSRNOG00000013195
Entrez:   65137
UniGene:   Rn.86410
Chromosome Mapping:   4q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.840000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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