PrimePCR™ SYBR® Green Assay: Phox2a, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005940
Assay Design:   exonic
Chromosome Location:   1:173085974-173086810question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000026702

Gene Information

regulates transcription of the dopamine beta-hydroxylase and tyrosine hydroxylase genes, may play a role in neurotransmitter metabolism [RGD, Feb 2006]

Gene Symbol:   Phox2a
Gene Name:   Paired mesoderm homeobox protein 2A
Aliases:   Not Available
RefSeq:   NM_053869
Ensembl:   ENSRNOG00000019706
Entrez:   116648
UniGene:   Rn.2858
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.090000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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