PrimePCR™ SYBR® Green Assay: Lhx3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005674
Assay Design:   exonic
Chromosome Location:   3:9018485-9019354question
Amplicon Length:   154
Splice Variants Targeted:   ENSRNOT00000025047 ENSRNOT00000045587 ENSRNOT00000007978 ENSRNOT00000026941 ENSRNOT00000003871

Gene Information

homeodomain containing transcription factor involved in motor neuron specification and pituitary development [RGD, Feb 2006]

Gene Symbol:   Lhx3
Gene Name:   Protein Lhx3; RCG45383, isoform CRA_a
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000018427
Entrez:   170671
UniGene:   Rn.198623
Chromosome Mapping:   3p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.340000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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