PrimePCR™ SYBR® Green Assay: Gipr, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005589
Assay Design:   exonic
Chromosome Location:   1:81336951-81337630question
Amplicon Length:   67
Splice Variants Targeted:   ENSRNOT00000021456

Gene Information

receptor for gastric inhibitory polypeptide/glucose-dependent insulinotropic peptide; induces cAMP production; mediates increase in intracellular calcium ions [RGD, Feb 2006]

Gene Symbol:   Gipr
Gene Name:   Gastric inhibitory polypeptide receptor
Aliases:   Not Available
RefSeq:   NM_012714
Ensembl:   ENSRNOG00000015860
Entrez:   25024
UniGene:   Rn.9676
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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