PrimePCR™ SYBR® Green Assay: Mst1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005300
Assay Design:   exonic
Chromosome Location:   8:116214828-116215020question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000026711

Gene Information

ligand for receptor tyrosine kinase STK/RON; may mediate germ cell-germ cell interaction during spermatogenesis, and acquisition of sperm motility and/or fertilizing capacity in the epididymis [RGD, Feb 2006]

Gene Symbol:   Mst1
Gene Name:   Hepatocyte growth factor-like protein precursor
Aliases:   Not Available
RefSeq:   NM_024352
Ensembl:   ENSRNOG00000019680
Entrez:   24566
UniGene:   Rn.10613
Chromosome Mapping:   8q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.160000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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