PrimePCR™ SYBR® Green Assay: Slc2a8, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005270
Assay Design:   exonic
Chromosome Location:   3:17356978-17357090question
Amplicon Length:   83
Splice Variants Targeted:   ENSRNOT00000022327

Gene Information

may transport glucose out of the rough endoplasmic reticulum into the cytosol in response to glucose administration; may play a role in glucose homeostasis in hippocampal neurons [RGD, Feb 2006]

Gene Symbol:   Slc2a8
Gene Name:   Solute carrier family 2, facilitated glucose transporter member 8
Aliases:   Not Available
RefSeq:   NM_053494
Ensembl:   ENSRNOG00000022274
Entrez:   85256
UniGene:   Rn.12923
Chromosome Mapping:   3p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.120000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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