PrimePCR™ SYBR® Green Assay: Adra1d, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005158
Assay Design:   exonic
Chromosome Location:   3:130635937-130636055question
Amplicon Length:   89
Splice Variants Targeted:   ENSRNOT00000028877

Gene Information

adrenergic receptor showing high affinity for the antagonist WB4101 and agonists phenylephrine and methoxamine [RGD, Feb 2006]

Gene Symbol:   Adra1d
Gene Name:   Alpha-1D adrenergic receptor
Aliases:   Not Available
RefSeq:   NM_024483
Ensembl:   ENSRNOG00000021256
Entrez:   29413
UniGene:   Rn.11314
Chromosome Mapping:   3q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.520000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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