PrimePCR™ SYBR® Green Assay: Gja5, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005133
Assay Design:   exonic
Chromosome Location:   2:218667478-218667575question
Amplicon Length:   68
Splice Variants Targeted:   ENSRNOT00000023488

Gene Information

forms intercellular connoxin channels in gap junctions; mediates cell-cell communication [RGD, Feb 2006]

Gene Symbol:   Gja5
Gene Name:   Gap junction alpha-5 protein
Aliases:   Not Available
RefSeq:   NM_019280
Ensembl:   ENSRNOG00000017484
Entrez:   50563
UniGene:   Rn.88300
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.590000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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