PrimePCR™ SYBR® Green Assay: Tfe3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0004936
Assay Design:   exonic
Chromosome Location:   X:16359008-16360413question
Amplicon Length:   68
Splice Variants Targeted:   ENSRNOT00000012829 ENSRNOT00000029776 ENSRNOT00000049889

Gene Information

Description Not Available

Gene Symbol:   Tfe3
Gene Name:   Transcription factor E3
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000009605
Entrez:   317376
UniGene:   Rn.9231
Chromosome Mapping:   Xq13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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