PrimePCR™ SYBR® Green Assay: Dynll2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0004880
Assay Design:   exonic
Chromosome Location:   10:74837396-74837518question
Amplicon Length:   93
Splice Variants Targeted:   ENSRNOT00000012279

Gene Information

gephyrin-binding protein that plays a role in postsynaptic targeting of neurotransmitter receptors or scaffolding proteins [RGD, Feb 2006]

Gene Symbol:   Dynll2
Gene Name:   Dynein light chain 2, cytoplasmic
Aliases:   Not Available
RefSeq:   NM_080697
Ensembl:   ENSRNOG00000008921
Entrez:   140734
UniGene:   Rn.36362
Chromosome Mapping:   10q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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