PrimePCR™ SYBR® Green Assay: Pclo, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0004848
Assay Design:   exonic
Chromosome Location:   4:16627928-16628070question
Amplicon Length:   113
Splice Variants Targeted:   ENSRNOT00000008637 ENSRNOT00000007608 ENSRNOT00000039966

Gene Information

presynaptic low-affinity Ca(2+) sensor; may be involved in the assembly and function of presynaptic active zones [RGD, Feb 2006]

Gene Symbol:   Pclo
Gene Name:   Protein piccolo isoform 1
Aliases:   Not Available
RefSeq:   NM_020098 NM_001110797
Ensembl:   ENSRNOG00000005726
Entrez:   56768
UniGene:   Rn.53925
Chromosome Mapping:   4q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.690000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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