PrimePCR™ SYBR® Green Assay: Podxl, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0004644
Assay Design:   exonic
Chromosome Location:   4:58591853-58591990question
Amplicon Length:   108
Splice Variants Targeted:   ENSRNOT00000016991

Gene Information

inhibits cell-cell adhesion; may play a role in maintaining filtration in the glomerular epithelium [RGD, Feb 2006]

Gene Symbol:   Podxl
Gene Name:   Podocalyxin
Aliases:   Not Available
RefSeq:   NM_138848
Ensembl:   ENSRNOG00000012495
Entrez:   192181
UniGene:   Rn.162741
Chromosome Mapping:   4q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 37.960000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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