PrimePCR™ SYBR® Green Assay: St6gal1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: St6gal1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: St6gal1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0004461
Assay Design:   exonic
Chromosome Location:   11:79807559-79807651question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000012270 ENSRNOT00000035389 ENSRNOT00000002499 ENSRNOT00000044591 ENSRNOT00000002496 ENSRNOT00000024679 ENSRNOT00000047408 ENSRNOT00000051968

Gene Information

may be involved in cell-type specific terminal glycosylation [RGD, Feb 2006]

Gene Symbol:   St6gal1
Gene Name:   Beta-galactoside alpha-2,6-sialyltransferase 1 isoform 2
Aliases:   Not Available
RefSeq:   NM_001113344 NM_147205
Ensembl:   ENSRNOG00000001823
Entrez:   25197
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.860000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download