PrimePCR™ SYBR® Green Assay: Cdkn2a, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0004217
Assay Design:   exonic
Chromosome Location:   5:111793607-111793834question
Amplicon Length:   194
Splice Variants Targeted:   ENSRNOT00000066011

Gene Information

tumor suppressor that inhibits CDK4; inactivation or loss is associated with cellular transformation; may be involved in CNS degeneration following injury [RGD, Feb 2006]

Gene Symbol:   Cdkn2a
Gene Name:   Cyclin-dependent kinase inhibitor 2A
Aliases:   Not Available
RefSeq:   NM_031550
Ensembl:   ENSRNOG00000006664
Entrez:   25163
UniGene:   Rn.48717
Chromosome Mapping:   5q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.790000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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