PrimePCR™ Probe Assay: Mup17, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Mup17, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Mup17, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0062788
Assay Design:   Intron-spanning
Chromosome Location:   4:61592147-61593637question
Amplicon Length:   170
Splice Variants Targeted:   ENSMUST00000075973 ENSMUST00000107521 ENSMUST00000107517 ENSMUST00000079697 ENSMUST00000166098 ENSMUST00000074700 ENSMUST00000095058 ENSMUST00000163931 ENSMUST00000122381 ENSMUST00000118759 ENSMUST00000103012 ENSMUST00000084548 ENSMUST00000098047 ENSMUST00000098046 ENSMUST00000117932 ENSMUST00000072678 ENSMUST00000098042 ENSMUST00000125461 ENSMUST00000075206 ENSMUST00000095049 ENSMUST00000107483 ENSMUST00000107484 ENSMUST00000098040 ENSMUST00000080606 ENSMUST00000082287 ENSMUST00000074018 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Mup17
Gene Name:   Major urinary protein 17
Aliases:   Gm12557, MUP 17, OTTMUSG00000007480
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000096688
Entrez:   100039206
Chromosome Mapping:   4 B3|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.610000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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