PrimePCR™ Probe Assay: Serpina1b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0062740
Assay Design:   Intron-spanning
Chromosome Location:   12:103728344-103729323question
Amplicon Length:   153
Splice Variants Targeted:   ENSMUST00000095450 ENSMUST00000164454

Gene Information

Description Not Available

Gene Symbol:   Serpina1b
Gene Name:   Serine (or cysteine) preptidase inhibitor, clade A, member 1B
Aliases:   D12Ucla2, Dom2, PI2, Spi1-2
RefSeq:   NC_000078.6 NT_039551.8 NT_114988.1
Ensembl:   ENSMUSG00000071178
Entrez:   20701
UniGene:   Mm.439693
Chromosome Mapping:   12 E|12 51.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.820000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download