PrimePCR™ Probe Assay: AC160968.4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0062722
Assay Design:   Intron-spanning
Chromosome Location:   Y:3402458-3403405question
Amplicon Length:   115
Splice Variants Targeted:   ENSMUST00000171534 ENSMUST00000100360 ENSMUST00000179404 ENSMUST00000166474 ENSMUST00000179508 ENSMUST00000178531 ENSMUST00000169382 ENSMUST00000180310 ENSMUST00000180202

Gene Information

Description Not Available

Gene Symbol:   AC160968.4
Gene Name:   RNA-binding motif protein, Y chromosome, family 1 member A1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095948
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.570000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download