PrimePCR™ Probe Assay: Vmn2r9, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0062680
Assay Design:   Intron-spanning
Chromosome Location:   5:108846369-108847539question
Amplicon Length:   142
Splice Variants Targeted:   ENSMUST00000172140 ENSMUST00000170419 ENSMUST00000164875 ENSMUST00000095922 ENSMUST00000053253 ENSMUST00000170341 ENSMUST00000167133

Gene Information

Description Not Available

Gene Symbol:   Vmn2r9
Gene Name:   Vomeronasal 2, receptor 9
Aliases:   EG435864
RefSeq:   NC_000071.6 NT_109320.5
Ensembl:   ENSMUSG00000091624
Entrez:   435864
UniGene:   Mm.387745
Chromosome Mapping:   5 F|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.250000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download