PrimePCR™ Probe Assay: Sly, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Sly, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Sly, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0044096
Assay Design:   Intron-spanning
Chromosome Location:   Y:54662359-54670146question
Amplicon Length:   96
Splice Variants Targeted:   ENSMUST00000178112 ENSMUST00000179076 ENSMUST00000178403 ENSMUST00000177842 ENSMUST00000178380 ENSMUST00000179884 ENSMUST00000180170 ENSMUST00000180332 ENSMUST00000177713 ENSMUST00000180058 ENSMUST00000179551 ENSMUST00000178639 ENSMUST00000179672 ENSMUST00000179330 ENSMUST00000180133 ENSMUST00000179631 ENSMUST00000180249 ENSMUST00000177834 ENSMUST00000178213 ENSMUST00000179024 ENSMUST00000179480 ENSMUST00000179336 ENSMUST00000178751 ENSMUST00000177626 ENSMUST00000178063 ENSMUST00000179811 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Sly
Gene Name:   Sycp3 like Y-linked
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000096518
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 40.580000
Efficiency 82

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download