PrimePCR™ Probe Assay: Vmn2r35, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043971
Assay Design:   Intron-spanning
Chromosome Location:   7:7814941-7816863question
Amplicon Length:   194
Splice Variants Targeted:   ENSMUST00000170922 ENSMUST00000075108 ENSMUST00000094866 ENSMUST00000165921 ENSMUST00000173459 ENSMUST00000169683 ENSMUST00000170402 ENSMUST00000168807 ENSMUST00000086282 ENSMUST00000146278 ENSMUST00000142934 ENSMUST00000066317 ENSMUST00000166499 ENSMUST00000164845 ENSMUST00000171942 ENSMUST00000169206 ENSMUST00000174388 ENSMUST00000108554 ENSMUST00000072787 ENSMUST00000171523 ENSMUST00000165611 ENSMUST00000108550 ENSMUST00000086298 ENSMUST00000074943

Gene Information

Description Not Available

Gene Symbol:   Vmn2r35
Gene Name:   Vomeronasal 2, receptor 35
Aliases:   EG625353
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000096399
Entrez:   625353
UniGene:   Mm.469824
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 38.430000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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