PrimePCR™ Probe Assay: Rnaset2b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043849
Assay Design:   Intron-spanning
Chromosome Location:   17:6994629-6997925question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000089119 ENSMUST00000179728 ENSMUST00000097420

Gene Information

Description Not Available

Gene Symbol:   Rnaset2b
Gene Name:   Ribonuclease T2B
Aliases:   0610007O07Rik, 4833423A10Rik, 4930532K22Rik, C79750, RNASE6PL, Rnaset2
RefSeq:   NC_000083.6 NT_039636.8
Ensembl:   ENSMUSG00000094724
Entrez:   68195
UniGene:   Mm.181237
Chromosome Mapping:   17|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.590000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download