PrimePCR™ Probe Assay: Vmn2r21, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043784
Assay Design:   Intron-spanning
Chromosome Location:   6:123520913-123531862question
Amplicon Length:   106
Splice Variants Targeted:   ENSMUST00000073948 ENSMUST00000172199 ENSMUST00000163607 ENSMUST00000170808 ENSMUST00000172391 ENSMUST00000075095 ENSMUST00000162046 ENSMUST00000032238 ENSMUST00000077851 ENSMUST00000030102

Gene Information

Description Not Available

Gene Symbol:   Vmn2r21
Gene Name:   Vomeronasal 2, receptor 21
Aliases:   EG546912
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000093820
Entrez:   546912
UniGene:   Mm.469876
Chromosome Mapping:   6 F2|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.380000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download