PrimePCR™ Probe Assay: Zfp429, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best specificity

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043650
Assay Design:   Intron-spanning
Chromosome Location:   13:67396649-67399767question
Amplicon Length:   150
Splice Variants Targeted:   ENSMUST00000021997 ENSMUST00000172266 ENSMUST00000057070 ENSMUST00000181071 ENSMUST00000109732

Gene Information

Description Not Available

Gene Symbol:   Zfp429
Gene Name:   Zinc finger protein 429
Aliases:   2810487A22Rik, AI929863, Rsl2
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000078994
Entrez:   72807
UniGene:   Mm.303790
Chromosome Mapping:   13 B3|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 40.720000
Efficiency 80

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download