PrimePCR™ Probe Assay: Smok3a, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043620
Assay Design:   Intron-spanning
Chromosome Location:   5:138021538-138032409question
Amplicon Length:   180
Splice Variants Targeted:   ENSMUST00000085886 ENSMUST00000171498 ENSMUST00000100537 ENSMUST00000166206

Gene Information

Description Not Available

Gene Symbol:   Smok3a
Gene Name:   Sperm motility kinase 3A
Aliases:   Smok2b1
RefSeq:   NC_000071.6 NT_039315.8
Ensembl:   ENSMUSG00000075599
Entrez:   545814
UniGene:   Mm.349005
Chromosome Mapping:   5 G2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 39.070000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download