PrimePCR™ Probe Assay: DXBay18, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DXBay18, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DXBay18, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043513
Assay Design:   Intron-spanning
Chromosome Location:   X:73139582-73141640question
Amplicon Length:   194
Splice Variants Targeted:   ENSMUST00000030676 ENSMUST00000136474 ENSMUST00000025520 ENSMUST00000105902 ENSMUST00000105900 ENSMUST00000069620 ENSMUST00000165133 ENSMUST00000002291 ENSMUST00000064454 ENSMUST00000169834 ENSMUST00000088459 ENSMUST00000179117 ENSMUST00000080324 ENSMUST00000118022 ENSMUST00000067417 ENSMUST00000121312 ENSMUST00000034689 ENSMUST00000088987 ENSMUST00000161650 ENSMUST00000161220 ENSMUST00000160780 ENSMUST00000119118 ENSMUST00000088970 ENSMUST00000109155 ENSMUST00000063710 ENSMUST00000075483 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   DXBay18
Gene Name:   DNA segment, Chr X, Baylor 18
Aliases:   Not Available
RefSeq:   NC_000086.7 NT_039706.8
Ensembl:   ENSMUSG00000071745
Entrez:   574405
Chromosome Mapping:   X A7.3|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.120000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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